Phenotypic identification of Candida species and relative expression of candida drug resistance (CDR1) gene in fluconazole resistant and sensitive Candida albicans

Fluconazole Sensitivity and CDR1 Pattern in Candida

  • Muneeza Anwar M.Phil. student (Researcher), Department of Microbiology, University of Health Sciences, Lahore, Pakistan.
  • Sidrah Saleem Professor, Department of Microbiology, University of Health Sciences, Lahore, Pakistan
  • Shah Jahan Associate professor, Department of Immunology, University of Health Sciences, Lahore ,Pakistan.
  • Muhammad Roman PhD student, Department of Microbiology, University of Health Sciences, Lahore, Pakistan.
  • Ayesha Ghazal Lecturer, Department of Microbiology, University of Health Sciences, Lahore, Pakistan.
  • Muhammad Usman Arshad Lab manager, Department of Microbiology, University of Health Sciences, Lahore, Pakistan.
DOI: https://doi.org/10.37723/jumdc.v14i4.856
Keywords: Candida Drug Resistant Gene 1, Polymerase Chain Reaction.

Abstract

ABSTRACT:

BACKGROUND & OBJECTIVE: Candida species belong to the genus Candida. It is a commensal yeast in healthy people and may cause life-threatening infections in people with impaired immune systems.The objective of the current study was to observe the frequency of Candida species, their resistance against fluconazole, and the upregulation of the candida drug-resistant gene in both fluconazole-sensitive and resistant isolates.


METHODOLOGY: A comparative study with 206 Candida species was collected on Sabouraud Dextrose agar from Jinnah Hospital from 2016-2018. The isolates were re-confirmed by using various microbiological techniques.APIC 20AUX made identification up to the species level. Antifungal susceptibility testing of Candida species against fluconazole was determined by the broth microdilution method. Real-time PCR was carried out on the sensitive and resistant isolates to observe the part of the CDR1 gene against fluconazole resistance.

Published
2023-11-25
How to Cite
Muneeza Anwar, Sidrah Saleem, Jahan , S., Muhammad Roman, Ayesha Ghazal, & Arshad, M. U. (2023). Phenotypic identification of Candida species and relative expression of candida drug resistance (CDR1) gene in fluconazole resistant and sensitive Candida albicans. Journal of University Medical & Dental College, 14(4). https://doi.org/10.37723/jumdc.v14i4.856
Issue
Vol 14 No 4 (2023): October-December
Section
Original Article

Copyright (c) 2023 Journal of University Medical & Dental College

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.